Manipulation of flavonoid biosynthesis in plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2461058

The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants.More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3', 5' hydrolase (F3'5'H), flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.

L'invention concerne des acides nucléiques et des fragments d'acides nucléiques codant des séquences d'acides aminés pour des enzymes biosynthétiques de flavonoïde chez les plantes, ainsi que l'utilisation de ceux-ci pour la modification de la biosynthèse des flavonoïdes chez les plantes. Plus spécifiquement, l'enzyme biosynthétique de flavonoïde est choisie dans le groupe comprenant chalcone isomérase (CHI), chalcone synthase (CHS), chalcone réductase (CHR), dihydroflavonol 4-réductase (DFR), leucoanthocyanidine réductase (LCR), flavonoïde 3', 5' hydrolase (F3'5'H), flavanone 3-hydroxylase (F3H), flavonoïde 3'-hydroxylase (F3'H), phénylalanine ammonia-olyase (PAL) et vestitone réductase (VR), ainsi que des fragments et des variants actifs au niveau fonctionnel de celles-ci.

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