Manipulation of tocopherol levels in transgenic plants

Details Manipulation of tocopherol levels in transgenic plants Manipulation of tocopherol levels in transgenic plants

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/14 (2006.01) C12N 9/10 (2006.01) C12N 15/29 (2006.01) C12N 15/52 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2340857

Disclosed is a gene sequence which as been identified as encoding 2-methyl-6- phytylplastoquinol/2-6-solanylplastoquinol-9 methyltransferase from photosynthetic organisms. The enzyme 2-methyl-6- phytylplastoquinol/2-methyl-6-solanylplastoquinol-9 methyltransferase is a fundamental enzyme in the production of tocopherols and plastoquinols in higher plants 2-Methyl-6-phytylplastoquinol/2-methyl-6-solanylplastoquinol-9 methyltransferase is believed to be involved in regulating the relative amounts of the various tocopherols present in photosynthetic organisms. By introducing a genetic construct having 2-methyl-6-phytylplastoquinol/2-methyl-6-solanylplastoquinol-9 methyltransferase coding sequence placed under the control of a plant promoter in a plant, transgenic plants can be made having altered 2-methyl-6- phytylplastoquinol/2-methyl-6-solanylplastoquinol-9 methyltransferase expression, to effect changes in the tocopherol profile of the plant. Transgenic plants can be made that have altered tocopherol levels in their tissues.

L'invention concerne une séquence de gène codant pour la 2-méthyl-6-phytylplastoquinol/2-méthyl-6-solanylplastoquinol-9-méthyltransférase d'organismes photosynthétiques. Le 2-méthyl-6-phytylplastoquinol/2-méthyl-6-solanylplastoquinol-9 méthyltransférase est une enzyme fondamentale dans la production de tocophérols et de plastoquinols chez les végétaux supérieurs. On pense qu'elle 2-méthyl-6-phytylplastoquinol/2-méthyl-6-solanylplastoquinol-9 méthyltransférase contribue à réguler les quantités relatives des divers tocophérols présents dans les organismes photosynthétiques. En introduisant chez une plante une construction génique ayant une séquence codant pour la 2-méthyl-6-phytylplastoquinol/2-méthyl-6-solanylplastoquinol-9-méthyltransférase, placée sous le contrôle d'un promoteur végétal, on peut obtenir des plantes transgéniques chez lesquelles l'expression de la 2-méthyl-6-phytylplastoquinol/2-méthyl-6-solanylplastoquinol-9 méthyltransférase, a été modifiée de façon à provoquer des changements dans le profil des tocophérols de la plante. On peut obtenir des plantes transgéniques permettant des modifications du taux de tocophérols tissulaire.

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