Mannitol induced promoter systems in bacterial host cells

Details Mannitol induced promoter systems in bacterial host cells Mannitol induced promoter systems in bacterial host cells

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2610405

The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.

L'invention concerne des procédés permettant de produire des peptides recombinants dans un hôte bactérien en utilisant un promoteur inductible par le mannitol, l'arabitol, le glucitol, ou le glycérol. La cellule bactérienne hôte qui produit le peptide a été rendue incapable de dégrader ou de métaboliser le mannitol, l'arabitol, ou le glucitol, ou des dérivés ou analogues de ceux-ci. L'invention concerne des cellules bactériennes qui ont été modifiées génétiquement de manière à inhiber le métabolisme ou la dégradation du mannitol, du glucitol, ou de l'arabitol, ou des dérivés ou analogues de ceux-ci. On utilise le mannitol, l'arabitol, le glucitol, ou le glycérol pour induire l'expression d'un polypeptide cible à partir d'un promoteur inductible, ce qui permet d'utiliser un inducteur de source de carbone stable et peu coûteux dans les processus de fermentation pour la production de peptides recombinants.

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