Mannose binding lectin and uses thereof

A - Human Necessities – 61 – K

Patent

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Details

A61K 38/17 (2006.01) A61K 38/08 (2006.01) A61P 31/04 (2006.01) A61P 31/12 (2006.01) A61P 33/00 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2483441

The present inventors have shown that MASP-depleted MBL is able to recruit MASPs from plasma and successfully activate the complement cascade. Furthermore, it has been discovered that MBL purified as a complex has limited ability to activate the complement cascade when compared to MASP-depleted MBL. Accordingly, the present invention provides a pharmaceutical composition comprising an isolated non-recombinant mannose binding lectin (MBL) substantially free from activated MBL associated serine proteases (MASPs) together with a pharmaceutically acceptable carrier or diluent. Also provided is a method of treating a subject in need of MBL comprising administering to the subject an effective amount of a pharmaceutical composition of the invention.

Cette invention a démontré que la lectine de fixation du mannose (MBL) appauvrie en sérine-protéases associées à la lectine MBL (MASP) est capable de recruter des sérine-protéases MASP dans le plasma et d'activer avec succès la cascade du complément. On a en outre découvert que la lectine MBL purifiée sous la forme d'un complexe à une capacité limitée à activer la cascade du complément par rapport à de la lectine MBL appauvrie en MASP. Ainsi, cette invention propose une composition pharmaceutique contenant de la lectine de fixation du mannose (MBL) non recombinée isolée, essentiellement exempte de sérine-protéases associées à la lectine MBL (MASP) activées, ainsi qu'à un excipient ou diluant acceptable sur le plan pharmaceutique. Cette invention concerne également un procédé servant à traiter un sujet nécessitant de la lectine MBL et consistant à lui administrer une quantité efficace d'une composition pharmaceutique de cette invention.

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