C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.235
C12N 15/23 (2006.01) A61K 38/21 (2006.01) A61P 31/12 (2006.01) A61P 37/04 (2006.01) C07K 14/57 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1341561
Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences in excess of about 200 base pairs in length, which sequences may comprise entire structural genes. Primarily disclosed are sequences for manufactured genes encoding, and a method for producing human immune interferon. Novel sequences are prepared from two or more DNA subunits provided with terminal regions comprising restriction endonuclease cleavage sites facilitating insertion of subunits into a selected vector for purposes of amplification during the course of the total assembly process. The total, finally-assembled sequences include at least one, and preferably two or more, unique restriction endonuclease cleavage site(s) at intermediate positions along the sequence, allowing for easy excision and replacement of subunits and the correspondingly facile prepara-tion of multiple structural analogs of polypeptides coded for by the sequences. Manufactured genes preferably include codons selected from among alternative codons specifying the same amino acid on the basis of preferential expression in a pro-jected host microorganism (e.g., E. coli) to be transformed. Illustrated is the preparation and expression of manufactured genes capable of directing synthesis of human immune and leukocyte interferons and of other biologically active protein-aceous products, which products differ from naturally-occurring forms in terms of the identity and/or relative position of one or more amino acids, and in terms of one or more biological and pharmacological properties but which substantially retain other such properties.
462319
Alton Norman K.
Peters Mary A.
Snitman David L.
Stabinsky Yitzhak
Amgen
Intermune Inc.
Ridout & Maybee Llp
LandOfFree
Manufacture and expression of large structural genes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Manufacture and expression of large structural genes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Manufacture and expression of large structural genes will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1319906