Manufacture of xylonic acid

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 7/58 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2755865

The invention provides a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is sponta-neously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the al-dose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase en-coding gene may result in higher acid concentrations. The method is especially suitable for producing xylonic acid from a hemi-celluiose hydrolysate such as hydrolysed lignocellulosic plant biomass.

L'invention porte sur un procédé de production d'acide xylonique à partir de xylose avec une souche fongique recombinante qui est génétiquement modifiée pour exprimer un gène de xylose déshydrogénase, qui est capable de convertir le xylose en xylonolactone, qui est hydrolysé spontanément ou par voie enzymatique en acide xylonique. L'acide xylonique est excrété à l'extérieur de la cellule hôte. La production de xylonate peut être couplée à la production de xylitol. En variante, si la production de xylitol n'est pas souhaitée, sa production est réduite par élimination de l'enzyme aldose réductase (ou xylose réductase spécifique), qui convertit le xylose en xylitol. L'expression d'un gène codant pour une lactonase hétérologue peut conduire à des concentrations supérieures en acide. Le procédé est particulièrement approprié pour produire de l'acide xylonique à partir d'un hydrolysat d'hémicellulose tel qu'une biomasse végétale lignocellulosique hydrolysée.

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