C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/078 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) C12N 1/04 (2006.01)
Patent
CA 2660518
Disclosed is a method for preparing activated lymphocytes, which comprises isolating lymphocytes from peripheral blood and proliferating and activating the isolated lymphocytes in vitro. According to the disclosed method, highly effective toxic cells can be prepared in large amounts by culturing human peripheral lymphocytes in the presence of an anti-CD3 antibody, IFN-.gamma. and IL-2. The activated lymphocytes proliferated according to the disclosed preparation method comprise both CD3-CD56+ (natural killer cell marker) cells that are the main components of LAK cells, and CD3+CD56+ cells that are the main components of CIK cells, and can be cultured in large amounts. Thus, the lymphocyte cells can show a significantly higher anticancer effect compared to when the LAK cells and the CIK cells are used alone.
La présente invention concerne un procédé permettant de préparer des lymphocytes activés, lequel procédé consiste à isoler des lymphocytes du sang périphérique et à développer et activer in vitro les lymphocytes isolés. Ce procédé permet de préparer de grandes quantités de cellules toxiques hautement efficaces par mise en culture de lymphocytes périphériques humains, en présence d'un anticorps anti-CD3, d'interféron .gamma. et d'IL-2. Les lymphocytes activés ainsi développés comportent à la fois des cellules CD3-CD56+ (marqueur de cellules tueuses naturelles) qui sont les principaux composants de cellules LAK, et des cellules CD3+CD56+ qui sont les principaux composants de cellules CIK, et peuvent être mis en culture dans de grandes quantités. Les cellules lymphocytes selon cette invention peuvent ainsi présenter un effet anticancéreux bien plus important que lorsque les cellules LAK et les cellules CIK sont utilisées seules.
An Kyung Chool
Ban Jung Hwa
Choi Sung Hee
Jang Jeong Su
Kang Chi Dug
Binex Co. Ltd.
Gowling Lafleur Henderson Llp
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