Marek's disease virus genes and their use in vaccines for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/38 (2006.01) A61K 31/70 (2006.01) A61K 39/255 (2006.01) A61K 39/295 (2006.01) A61K 48/00 (2006.01) C07K 14/055 (2006.01) C12N 7/01 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2226362

A nucleotide sequence encoding the gp82 polypeptide of Marek's disease virus is disclosed. Also disclosed are recombinant viruses which are useful as vaccines for protecting agaisnt Marek's Disease, preferably containing two or more genes encoding Marek's Diesease Virus antigens such as glycoprotein B and glycoprotein gp82, under the control of a poxvirus promoter within a region of the DNA of fowlpox virus which is not essential for virus growth. Also provided is a vaccine exhibiting a synergistic immunoprotective effect, comprising a recombinant fowlpox virus expressing Marek's Disease Virus gB protein in combination with turkey herpesvirus. A method of immunizing poultry, comprising administering any of the disclosed vaccines, is also provided.

L'invention concerne une séquence de nucléotides qui code le polypeptide gp82 du virus de la maladie de Marek, ainsi que des virus recombinants utiles comme vaccins qui protègent contre la maladie de Marek et qui contiennent de préférence deux ou plusieurs gènes qui codent des antigènes du virus de la maladie de Marek tels que la glycoprotéine B et la glycoprotéine gp82, sous la régulation d'un promoteur du poxvirus situé dans une région de l'ADN de l'avipoxvirus qui n'est pas essentielle à la croissance du virus. L'invention concerne également un vaccin qui présente un effet immunoprotecteur synergique et qui contient un avipoxvirus recombinant qui exprime la protéine gB du virus de la maladie de Marek associé au virus de l'herpès du dindon, ainsi qu'un procédé d'immunisation de gallinacés qui comprend l'administration d'un des vaccins décrits.

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