Marked peptides having affinity for a phospholipid and uses

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01) A61K 51/08 (2006.01) A61P 7/02 (2006.01) A61P 29/00 (2006.01) C07D 401/06 (2006.01) C07D 401/12 (2006.01) C12N 15/12 (2006.01) G01N 33/58 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2491344

The invention concerns a peptide marked by Fluor 18 for specific identification of lipid vectors. The inventive peptide comprises the following peptide sequence (PI): J¿1?-J¿2?-J¿3?-J¿4?-J¿5?-J¿6?-Z¿7?-U¿8?-J¿9?-J¿10?-U¿11?-Arg-J¿13?-J¿14?-U¿15?-Lys-Gly-X¿18?-Gly-Thr-J¿21?-Glu-J¿23?-J¿24?-U¿25?-J¿26?-J¿27?-J¿28?-U¿29?-J¿30?-J¿31?-Arg-J¿33?-J¿34?-J¿35?-J¿36?-B¿37?-J¿38?-J¿39?-U¿40?-J¿41?-J¿42?-J¿43?-U¿44?-J¿45?-J¿46?-J¿47?-J¿48?-J¿49?-Arg-J¿51?-U¿52?-J¿53?-J¿54?-Asp-U¿56?-Lys-Ser-Z¿59?-Leu-J¿61?-J¿62?-J¿63?-J¿64?-Z¿65?-J¿66?-J¿67?-U¿68?-J¿69?-J¿70?-J¿71?-U¿72?-J¿73?-J¿74?-J¿75? (PI) wherein the amino acids J are selected independently of one another among Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Lys, Orn, Pro, Ser, Thr and Tyr; the amino acids U are selected independently of one another among Ala, Cys, Gly, Ile, Leu, Met, Phe, Trp, Tyr et Val; the amino acid X?18¿¿ ?is selected independently of the other amino acids of the sequence among Ala, Asn, Cys, Gln, Gly, His, Ile, Leu, Met, Phe, Ser, Thr, Trp, Tyr et Val; the amino acid B?37¿ is selected independently of the other amino acids of the sequence Arg, Ala, Cys, Gly, Ile, Leu, Met, Phe, Trp, Tyr et Val; the amino acid? ¿Z?7¿ is selected independently of the other amino acids among Asp or Glu; the amino acids¿ ?Z?59¿¿ ?and Z?65¿ are selected among Glu, Asp, Lys or Arg; the exponents of residues J, Z, U, X and B represent the position of the amino acids in said sequence.

La présente invention se rapporte à un peptide marqué par le Fluor 18 pour la reconnaissance spécifique de vecteurs lipidiques. Le peptide de l'invention comprend la séquence peptidique (PI) suivante : J1-J2-J3-J4-J5-J6-Z7-U8-J9-J10- U11-Arg-J13-J14-U15-Lys-Gly-X18-Gly-Thr-J21-Glu-J23-J24-U25-J26-J27-J28-U29- J30-J31-Arg-J33-J34-J35-J36-B37-J38-J39-U40-J41-J42-J43-U44-J45-J46-J47-J48- J49-Arg-J51-U52-J53-J54-Asp-U56-Lys-Ser-Z59-Leu-J61-J62-J63-J64-Z65-J66-J67- U68-J69-J70-J71-U72-J73-J74-J75 (PI)dans laquelle les acides aminés J sont choisis indépendamment les uns des autres parmi les acides aminés essentiels, ou des dérivés de ceux-ci, de telle manière que au moins 50% d'entre eux sont des résidus polaires choisis parmi Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Lys, Orn, Pro, Ser, Thr et Tyr ; les acides aminés U sont choisis indépendamment les uns des autres parmi Ala, Cys, Gly, Ile, Leu, Met, Phe, Trp, Tyr et Val ; l'acide aminé X18 est choisi indépendamment des autres acides aminés de la séquence parmi Ala, Asn, Cys, Gln, Gly, His, Ile, Leu, Met, Phe, Ser, Thr, Trp, Tyr et Val ; l'acide aminé B37 est choisi indépendamment des autres acides aminés de la séquence parmi Arg, Ala, Cys, Gly, Ile, Leu, Met, Phe, Trp, Tyr et Val ; l'acide aminé Z7 est choisi indépendamment des autres acides aminés parmi Asp ou Glu ; les acides aminés Z59 et Z65 sont choisis parmi Glu, Asp, Lys ou Arg ; les exposants des résidus J, Z, U, X et B représentant la position de ces acides aminés dans ladite séquence.

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