C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 17/02 (2006.01) C12N 5/04 (2006.01)
Patent
CA 2264270
The present invention relate to a method to increase the production level of paclitaxel by changing the temperature during the plant cell culture. According to the present invention, the production of the paclitaxel comprises the following procedure: (i) cultivating the Taxus genus plant cells in a medium at ca. 20 to 25 ~C; and (ii) when growth of the plant cells has progressed sufficiently, changing the cultivation temperature to ca. 26 to 32 ~C to continue the culture. The present invention comprises also the method of increasing the paclitaxel production by inoculating the cells at a high initial concentration and by increasign the saccharide concentration in the medium. According to the present invention, paclitaxel can be mass-produced conveniently and therefore has an industrial application.
L'invention concerne un procédé qui permet d'augmenter la production de paclitaxel, en modifiant la température pendant la culture des cellules de plante. Selon la présente invention, la production du paclitaxel comprend les étapes suivantes: (i) culture des cellules végétales du genre Taxus dans un milieu à environ 20-25 ·C; et, (ii) après une croissance suffisamment avancée des cellules, modification de la température de culture, qui passe à environ 26-32 ·C, afin de poursuivre le processus. L'invention concerne aussi un procédé qui permet d'augmenter la production de paclitaxel en inoculant les cellules à une concentration initiale élevée et en accroissant la concentration de saccharide dans le milieu. Il découle de l'invention que l'on peut convenablement produire en série le paclitaxel, d'où une possibilité d'application industrielle.
Chang Moon-Seok
Choi Eunsoo
Choi Ho-Joon
Choi Hyung-Kyoon
Hong Seung-Suh
Goudreau Gage Dubuc
Samyang Genex Corporation
LandOfFree
Mass production of paclitaxel by changing the temperature of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mass production of paclitaxel by changing the temperature of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mass production of paclitaxel by changing the temperature of... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1376706