Mass spectrometric analysis of biopolymers

H - Electricity – 01 – J

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H01J 49/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G06F 17/00 (2006.01)

Patent

CA 2420567

The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separatioln technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.

L'invention concerne l'utilisation d'étiquettes uniques d'un biopolymère spécifique que l'on peut exploiter pour déterminer la concentration du biopolymère dans des solutions brutes. En mode de réalisation préféré, le biopolymère est une protéine ou un polynucléotide. L'invention concerne en particulier un procédé permettant de déterminer et de quantifier des biomolécules dans des mélanges bruts, par séparation et spectrométrie de masse combinées. Selon une variante générale, on sélectionne une biomolécule cible aux fins d'analyse et on établit un analogue de cette biomolécule. Une intégration de la surface des pics pour les paires de peptides fournit une mesure directe de la quantité de protéine cible dans la solution brute.

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