Mass spectrometry assay for eif4e and eif4e regulon activity

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 5/00 (2006.01)

Patent

CA 2703804

Provided is a highly sensitive high throughput mass spectrometry -based quantitative assay for 4E/4E regulon pathway proteins has been developed which provides for single sample multiplexed analysis, as well as the analysis of protein phosphorylation states. It may be adapted for use as the first single sample analytical method of the 4E/4E regulon biological pathway.

L'invention concerne une analyse par spectrométrie de masse de l'activité du eLF4E et du régulon eLF4E. Une analyse quantitative basée sur spectrométrie de masse à fort rendement et à sensibilité élevée des protéines à voie 4E/régulon 4E a été développée, permettant une analyse multiplexée d'un seul échantillon, ainsi que l'analyse des états de phosphorylation de protéines. Elle peut être adaptée pour une utilisation en tant que premier procédé analytique d'échantillon unique de la voie biologique 4E/régulon 4E.

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