Mass spectrometry assay for plasma-renin

G - Physics – 01 – N

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G01N 27/00 (2006.01) G01N 1/34 (2006.01) G01N 33/483 (2006.01) G01N 33/68 (2006.01) H01J 49/26 (2006.01)

Patent

CA 2732109

Provided are methods for measuring renin activity in a plasma sample using mass spectrometry. The methods generally involve ionizing purified angiotensin 1 from the sample and detecting the amount of angiotensin 1 ions generated. The amount of detected angiotensin 1 ions are then related to the amount of angiotensin 1 generated in the sample, which in turn is related to renin activity in the sample.

L'invention concerne des procédés pour mesurer une activité de rénine dans un échantillon de plasma à laide dune spectrométrie de masse. Les procédés entraînent, de manière générale, l'ionisation d'angiotensine 1 purifiée provenant de l'échantillon et la détection de la quantité d'ions d'angiotensine 1 générés. La quantité d'ions d'angiotensine 1 détectés est ensuite mise en rapport avec la quantité d'angiotensine 1 générée dans l'échantillon, qui est à son tour mise en rapport avec l'activité de rénine dans l'échantillon.

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