C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 19/20 (2006.01) C07H 19/073 (2006.01) C07H 19/10 (2006.01) C07H 19/173 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) H01J 49/26 (2006.01) C12P 19/34 (2006.01) C40B 30/00 (2006.01) C40B 50/14 (2006.01)
Patent
CA 2425112
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the -OH group at the 3'-position of the deoxyribose.
L'invention concerne des méthodes permettant de fixer un acide nucléique sur une surface solide et de séquencer un acide nucléique par détection de l'identité de chaque analogue nucléotidique après l'incorporation de l'analogue nucléotidique dans un brin d'ADN en développement au cours d'une réaction polymérasique. L'invention concerne également des analogues nucléotidiques comportant des marqueurs uniques fixés par l'intermédiaire d'un lieur clivable, ainsi qu'un groupe chimique clivable servant à coiffer le groupe OH en position 3' du désoxyribose.
Edwards John Robert
Itagaki Yasuhiro
Ju Jingyue
Li Zengmin
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
The Trustees Of Columbia University In The City Of New York
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