G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2622743
The subject invention concerns enzyme-based methods for detecting and assaying for glyoxylate. In particular, the invention is directed to methods for assaying for glyoxylate produced by the reaction of peptidylglycine .alpha.- amidating monooxygenase (PAM). The subject invention also concerns methods for assaying for the enzyme peptidylglycine .alpha.- amidating monooxygenase. The detection of glyoxylate using the present invention is indicative of the presence of PAM. The subject invention also concerns methods for screening for peptide hormones and any N-acyl-glycine or N-aryl-glycine conjugated molecule.
L'invention concerne des méthodes faisant intervenir des enzymes pour la détection et l'analyse de glyoxylate. Elle concerne plus particulièrement des méthodes d'analyse du glyoxylate obtenu par la réaction de peptidylglycine a-amidating monooxygénase (PAM). L'invention concerne également des méthodes d'analyse de l'enzyme peptidylglycine a- amidating monooxygénase. La détection de glyoxylate au moyen de cette invention renseigne sur la présence de PAM. Sont également décrites des méthodes de criblage d'hormones peptidique et de tout molécule conjuguée N-acyl-glycine ou N-aryl-glycine.
Carpenter Sarah E.
Merkler David J.
Mbm Intellectual Property Law Llp
University Of South Florida
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