Materials and methods for assaying for methylation of cpg...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2629008

Provided are methods, reagents, and kits for evaluating cancer, such as prostate cancer, in a subject. Disclosed methods of evaluating cancer include methods of diagnosing cancer, methods of prognosticating cancer and methods of assessing the efficacy of cancer treatment. The methods include assaying a biological sample for methylation of a CpG island associated with specified genes. Provided reagents and kits include primers suitable for amplifying at least a portion of a target CpG islands associated with specified genes.

La présente invention concerne des procédés, des réactifs et des trousses pour l'évaluation d'un cancer, comme le cancer de la prostate, chez un sujet. Les procédés d'évaluation d'un cancer décrits comprennent des procédés pour diagnostiquer un cancer, des procédés pour pronostiquer un cancer et des procédés pour évaluer l'efficacité d'un traitement contre un cancer. Les procédés comprennent le dosage d'un échantillon biologique pour la méthylation d'un ilot CpG associé à des gènes spécifiés. L'invention concerne des réactifs et des trousses qui comprennent des amorces appropriées pour amplifier les ilots CpG cibles associés à des gènes spécifiés.

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