C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12Q 1/04 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2269657
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns methods for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.
La présente invention concerne l'utilisation de la formyl-CoA transférase en association avec l'oxalyl-CoA décarboxylase pour tester et mesurer l'oxalate dans des échantillons biologiques. Le système d'enzymes de l'invention permet de convertir l'oxalate en dioxyde de carbone et en formiate. La production de formiate étant directement liée à la teneur de l'échantillon en oxalate, en évaluant la teneur résultante en formiate, on a la possibilité d'évaluer également de façon précise, sensible et rapide des teneurs, mêmes faibles, en oxalate. L'invention concerne également le clonage, le séquençage et l'expression des gènes codant pour la formyl-CoA transférase et l'oxalyl-CoA décarboxylase d'Oxylobacter formigenes. L'invention, qui concerne aussi des procédés de détecter dans un échantillon la présence d'organismes du genre Oxylobacter formigenes, concerne enfin les sondes et amorces de polynucléotides intervenant pour le procédé de détection.
Peck Ammon B.
Sidhu Harmeet
Macrae & Co.
University Of Florida
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