Materials and methods for identifying and using yeast...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/16 (2006.01) C12P 1/02 (2006.01) C12P 7/10 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2753538

Disclosed herein are materials and methods for creating and/or isolating variants of yeasts especially variants of Saccharomyces cerevisiae that can grow on sugars other than D-glucose in the presence of amounts of 2-deoxy-glucose and or D- glucose that inhibit most strains of yeast from growing on sugars other than D- glucose. Selection media that can be used to isolate such variants include pentose sugars such as D-xylose, L-glutamine and 2-deoxy- glucose. Mutations in the Grr1 and Red genes in some strains also produce variants that can grow on sugars including the pentose D-xylose in the presence of 2-deoxy-glucose.

L'invention concerne des matériaux et des procédés de création et/ou d'isolation de variants de levures, en particulier des variants de Saccharomyces cerevisiae qui peuvent croître sur des sucres autres que le dextrose en présence de certaines quantités de 2-désoxyglucose et/ou de dextrose qui empêchent la majeure partie des souches de levures de croître sur des sucres autres que le dextrose. Les milieux de sélection qui peuvent être utilisés pour isoler de tels variants comprennent les sucres pentoses tels que le D-xylose, la L-glutamine et le 2-désoxyglucose. Des mutations des gènes Grr1 et Red de certaines souches produisent également des variants pouvant croître sur les sucres tels que la pentose D-xylose en présence de 2-désoxyglucose.

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