Materials and methods for increasing corn seed weight

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2264481

The subject invention pertains to novel variants of the maize gene, Shrunken2(Sh2) and a method of using that gene. The variant gene, Sh2-m1Rev6, encodes a subunit of the ADP-glucose pyrophosphorylase (AGP) enzyme that has additional amino acids inserted in or near the allosteric binding site of the protein. Corn seed expressing the Sh2-m1Rev6 gene has a 15 % weight increase over wild type seed. The increase in seed weight is not associated simply with an increase in percentage starch content of the seed.

L'invention porte sur de nouvelles variantes du gène de maïs Shrunken 2 (Sh2) et sur son procédé d'utilisation. La variante Sh2-mlRev6 code pour une sous-unité de l'enzyme ADP-glucose pyrophosphorylase (AGP) qui comporte des acides aminés insérés dans le site allostérique de fixation de la protéine ou à proximité. Les graines de maïs exprimant le gène Sh2-mlRev6 ont un poids dépassant de 15 % celui des graines sauvages. Cet accroissement de poids n'est pas simplement lié à un accroissement du pourcentage d'amidon contenu dans la graine.

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