Materials and methods for releasing genetic material

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2480566

The invention provides methods for isolating and releasing genetic material from a solid medium, such as a DNA array, a filter, a card, or a multiple-well plate, using a wash solution comprising a photolytic agent, followed by subsequent exposure to a light source to release the genetic material, such as genomic DNA. The invention also includes devices and kits for practicing these methods.

Cette invention concerne des méthodes visant à isoler et à libérer du matériel génétique à partir d'un milieu solide tel qu'une matrice d'ADN, un filtre, une carte ou une plaque à cupules multiples, au moyen d'une solution de lavage renfermant un agent photolytique et exposition subséquente à une source lumineuse pour libérer le matériel génétique, de l'ADN génomique par exemple. L'invention concerne également des dispositifs et des kits permettant de mettre ces méthodes en oeuvre.

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