Materials and methods for the efficient production of pasteuria

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 3/00 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C12N 1/20 (2006.01) C12P 1/04 (2006.01)

Patent

CA 2379601

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria, in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria spores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

L'invention concerne des nouvelles techniques utiles pour cultiver des bactéries. Ces techniques sont notamment utiles pour cultiver des bactéries parasites in vitro en l'absence de tissu hôte. Dans un mode de réalisation de la présente invention, des spores de Pasteuria, telles que les spores qui infectent le nématode cécidogène Meloidogyne arenaria ou d'autres nématodes hôtes, sont cultivées in vitro. Le procédé de la presente invention est très utile du fait que la Pasteuria peut être cultivée en l'absence de tissu de nématode.

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