C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2425904
The invention provides improved methods for determining the methylation profile of a nucleic acid sequence and for determining one or more base changes in the target nucleic acid sequence as compared to a corresponding control sequence. The methods are one-step methods which can be incorporated with known amplification techniques such as PCR. The invention also provides methods for determining changes in nucleic acid sequences either via their methylation profile or owing to mutations of one or more bases.
La présente invention concerne des procédés améliorés permettant de déterminer le profil de méthylation d'une séquence d'acide nucléique et de déterminer un ou plusieurs changements de base dans la séquence d'acide nucléique cible comparée à une séquence témoin correspondante. Ces procédés sont des procédés à une étape qui peuvent être incorporés aux techniques d'amplification traditionnelles telles que la PCR. L'invention concerne également des procédés permettant de déterminer des changements dans les séquences d'acide nucléique soit à travers leur profil de méthylation, soit en raison des mutations d'une ou de plusieurs bases.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Cancer Research Technology Limited
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