Materials and methods relating to the increase in protein...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 38/18 (2006.01) A61K 38/19 (2006.01) C07K 14/705 (2006.01) C07K 14/785 (2006.01) C12N 5/10 (2006.01) A61K 38/00 (2006.01) A61K 38/11 (2006.01)

Patent

CA 2414342

The invention provides a protein framework which allows active polypeptides e.g. ligands or antigens to be displayed at increased concentration. The inventors show that the lectin binding domains of collectins can be replaced by a polypeptide of interest and that polypeptide can be multimerised by the framework of the collectin and as a result displayed in greater number on a single structure. The inventors show that the activity of polypeptides such as those of the TNF superfamily are significantly enhanced when displayed in this way.

L'invention concerne une charpente protéique qui permet à des polypeptides actifs, par ex. des ligands ou des antigènes, d'être présents dans une concentration augmentée. Les inventeurs montrent que les domaines liant la lectine de collectines peuvent être remplacés par un polypeptide et que ce polypeptide peut être multimérisé par la charpente de la collectine et en conséquence présent en plus grand nombre sur une structure unique. Les inventeurs montrent également que l'activité de polypeptides tels que ceux de la superfamille TNF sont significativement améliorés lorsqu'ils sont ainsi regroupés.

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