Materials and methods to enhance hematopoietic stem cells...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/87 (2006.01) C12N 5/0789 (2010.01) A01N 65/00 (2009.01) A61K 31/405 (2006.01) A61K 31/5415 (2006.01) A61K 35/12 (2006.01) A61K 45/00 (2006.01) A61K 48/00 (2006.01) A61P 43/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2742876

This disclosure is directed to the methods of enhancing hematopoietic stem cells (HSPC) and progenitor cell (HSPC) engraftment procedure. Treatment in vivo of a HSPC donor with compounds that reduce PGE2 biosynthesis or PGE2 re-ceptor antagonists alone, or in combination with other hematopoietic mobilization agents such as AMD3100 and G-CSF, increas-es the circulation of available HSPCs. Compounds that reduce the cellular synthesis of PGE2 include non-steroidal anti-inflamma-tory compounds such as indomethacin. Treatment ex vivo of HSPC with an effective amount of PGE2 or at least one of its deriva-tives such as 16,16-dimethyl prostaglandin E2 (dmPGE2), promotes HSPC engraftment. Similar methods may also be used to in-crease viral-mediated gene transduction efficacy into HSPC.

Cette invention concerne des procédés d'amélioration de protocole de prise de greffe de cellules souches et de cellules progénitrices hématopoïétiques (CSPH). Le traitement in vitro d'un donneur de CSPH avec des composés qui réduisent la biosynthèse de PGE2 ou des antagonistes de récepteurs de PGE2, seuls ou en combinaison avec des agents de mobilisation hématopoïétique comme AMD3100 et G-CSF, augmente la circulation des CSPH disponibles. Les composés qui réduisent la synthèse cellulaire de PGE2 comprennent des composés anti-inflammatoires non stéroïdiens comme l'indométhacine. Le traitement ex vivo des CSPH avec une quantité efficace de PGE2 ou d'au moins un de ses dérivés comme la 16,16-diméthylprostaglandine E2 (dmPGE2), active la prise de greffe des CSPH. Des procédés similaires peuvent également être utilisés pour augmenter l'efficacité de transduction de gène à médiation virale dans des CSPH.

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