Matrix attachment regions (mars) for increasing...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2658775

Isolated and purified MAR sequences of human and non-human animal origin are disclosed as are nucleotide sequences corresponding to or based on them. In particular, MARs and MAR constructs with high transcription and/or protein production enhancing activities are disclosed and so are methods for identifying such MARs, designing such MAR constructs and employing them, e.g., for high yield production of proteins.

L'invention porte sur des séquences MAR isolées et purifiées d'origine humaine et animale, tout comme sur des séquences de nucléotides correspondantes ou basées sur celles-ci. En particulier, des MAR ou des constructions MAR avec des activités de transcription élevées et/ou des activités accrues de production de protéines sont divulguées, ainsi que des méthodes pour identifier de telles MAR, mettant en point de telles constructions MAR et les employant, par exemple, pour une production à haut rendement de protéines.

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