Maturation of somatic embryos

A - Human Necessities – 01 – H

Patent

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A01H 4/00 (2006.01)

Patent

CA 2323862

A method of developing and maturing somatic embryos in a growth environment, which method comprises manipulating the water availability of the growth environment using a physical means of control. The invention also provides a growth environment for maturing somatic embryos, wherein the water potential of the embryogenic tissue is manipulated to optimize somatic embryo development and maturation. The invention further relates to a somatic embryo matured by the method of the invention. In the invention, a physical means of control is used to affect the water potential of the embryogenic tissue and developing somatic embryos growth medium, rather than a chemical means such as the introduction of PEG, to stimulate the maturation of the embryos. The physical means may be operated, for example, by separating the somatic embryos from the growth medium by a porous support, or by introducing a gelling agent (e.g. gellan gum) into the growth medium in larger than normal quantities.

L'invention concerne une méthode de développement et de maturation d'embryons somatiques dans un environnement de croissance, la méthode consistant à manipuler l'hydraulicité de l'environnement de croissance grâce à un moyen de commande physique. L'invention concerne également un environnement de croissance permettant la maturation d'embryons somatiques, le potentiel hydrique des tissus embryogéniques étant manipulé pour optimiser le développement et la maturation d'embryons somatiques. L'invention concerne aussi un embryon somatique amené à maturation grâce à la méthode selon l'invention. Dans la méthode selon l'invention, on utilise un moyen physique de commande pour agir sur le potentiel hydrique des tissus embryogéniques et le milieu de croissance des embryons somatiques en développement, plutôt qu'un moyen chimique tel que l'introduction de PEG (polyéthylène glycol), afin de stimuler la maturation des embryons. On peut actionner le moyen physique, par exemple, en séparant les embryons somatiques du milieu de croissance au moyen d'un support poreux ou par introduction d'un agent gélifiant (par exemple de la gomme gellane) dans le milieu de croissance en quantité supérieure à la normale.

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