Mayfly centroptilum triangulifer as a bioassay organism

Details Mayfly centroptilum triangulifer as a bioassay organism Mayfly centroptilum triangulifer as a bioassay organism

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) G01N 33/18 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2111521

The invention provides a method of testing for the presence of any substance in an environment to which any life stage of a Centroptilum triangulifer mayfly is exposed and which has an effect on the mayfly comprising collecting a sample of Centroptilum triangulifer mayflies exposed to the substance to be tested, and determining the effect of the substance on the mayfly by at least one of (a) observing the mayfly for a lethal or sublethal effect and (b) sacrificing individual mayflies, and analyzing the sacrificed mayflies for at least one of (i) the presence of at least one of the substance and any of its metabolites, (ii) the quantity of at least one of the substance and any of its metabolites and (iii) a lethal or sublethal effect of at least one of the substance and any of its metabolites. In a preferred embodiment for testing for a substance and any of its metabolites, the substance and any metabolites being tested are extracted and separated from the sacrificed mayflies in a solvent mixture, and the substance and any metabolites are identified and quantified using appropriate equipment and techniques.

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