Mdck cell lines supporting viral growth to high titers and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/00 (2006.01) C12N 7/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2663522

The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.

La présente invention concerne de nouvelles cellules MDCK qui peuvent permettre de cultiver des virus, par exemple les virus de la grippe, dans une culture de cellules jusqu'à un titre plus élevé qu'il n'était possible jusqu'à maintenant. Les cellules MDCK peuvent être adaptées à un milieu de culture exempt de sérum. La présente invention concerne en outre des compositions de culture de cellules comprenant les cellules MDCK et des procédés de culture pour la croissance des cellules MDCK. La présente invention concerne en outre des procédés de production des virus de la grippe en culture de cellules utilisant les cellules MDCK de l'invention.

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