Meadowfoam-based bioherbicide products

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 13/00 (2006.01) A01N 65/08 (2009.01) A23L 1/36 (2006.01) A61K 8/97 (2006.01) A61K 36/185 (2006.01) C12P 1/00 (2006.01) C12P 13/02 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2693262

Particular aspects provide methods for converting glucosinolate in a glucosinolate- containing plant material to glucosinolate breakdown products (GBPs), comprising: providing an amount of processed glucosinolate-containing plant material, the processed material being depleted of oil and glucosinolate converting enzyme activity by virtue of said processing; providing an amount of glucosinolate converting enzyme activity; mixing the processed material with the amount enzyme activity; hydrating the mixture; and incubating the hydrated mixture, wherein the glucosinolates are enzymatically converted to GBPs. Preferably, the processed plant material comprises a oilseed-derived seedmeal material (e.g., meadowfoam seedmeal) from which the oil has been removed by the processing (e.g., solvent extraction and/or heat treatment). In particular embodiments, the glucosinolate converting enzyme activity comprises at least one of a myrosinase activity and a nitrile- forming activity. Additional aspects provide low-fat compositions (e.g., herbicide, fungicide, insecticide, bacteriostatic or bactericidal, cosmetic, cosmeceutical or pharmaceutical) comprising GBPs derived from a glucosinolate-containing plant material.

L'invention concerne des procédés de conversion de glucosinolate présent dans un matériau de plante contenant du glucosinolate en des produits de rupture de glucosinolate (GBP), comprenant : la fourniture d'une quantité de matériau de plante contenant du glucosinolate traité, le matériau traité étant diminué d'une activité d'enzyme de conversion d'huile et de glucosinolate en vertu dudit traitement ; la fourniture d'une quantité d'activité d'enzyme de conversion de glucosinolate ; le mélange du matériau traité avec la quantité d'activité d'enzyme ; l'hydratation du mélange ; et l'incubation du mélange hydraté, les glucosinolates étant enzymatiquement convertis en GBP. De préférence, le matériau de plante traité comprend un matériau de farine de graine dérivée de graines oléagineuses (par exemple, la farine de graine d'écume des prés) duquel l'huile a été retirée par le traitement (par exemple, extraction de solvant et/ou traitement thermique). Dans des modes de réalisation particuliers, l'activité d'enzyme de conversion de glucosinolate comprend au moins une parmi une activité de myrosinase et d'une activité de formation de nitrile. Des aspects supplémentaires fournissent des compositions à faible teneur en matière grasse (par exemple, herbicide, fongicide, insecticide, bactériostatique ou bactéricide, cosmétique, cosméceutique ou pharmaceutique) comprenant des GBP dérivés d'un matériau de plante contenant du glucosinolate.

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