C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01) C12N 15/13 (2006.01) C12N 15/63 (2006.01) C12N 15/79 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2589487
The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
La présente invention a trait à une molécule d'acide nucléique comprenant une séquence nucléotidique codant pour un polypeptide bifonctionnel comprenant un domaine de liaison à l'ADN d'une protéine appartenant à la famille de protéines de liaison méthyle-CpG (MBD) et la portion Fc d'un anticorps. L'invention a également trait à des vecteurs, des cellules hôtes comportant ladite molécule d'acide nucléique et des polypeptides codés par ladite molécule d'acide nucléique ainsi qu'à des procédés pour la production dudit polypeptide. L'invention a trait en outre à un anticorps de liaison spécifique audit polypeptide et à des compositions, notamment des compositions de diagnostic comportant la/les molécules, le(s) vecteur(s), la/les cellule(s) hôte(s), le(s) polypeptide(s) ou anticorps de la présente invention. L'invention a trait enfin à des procédés et des applications mettant en oeuvre les polypeptides de la présente invention pour la détection d'ADN méthylé, notamment dans du tissu tumoral ou dans des cellules tumorales.
Goudreau Gage Dubuc
Klinikum Der Universitat Regensburg
Sequenom Inc.
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