Means and methods for fibroblast-like or macrophage-like...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/861 (2006.01) A61K 48/00 (2006.01) A61P 19/02 (2006.01) C07K 14/075 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/34 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2364499

The invention provides a nucleic acid delivery vehicle with or having been provided with at least a tissue tropism for fibroblast-like or macrophage-like cells, preferably synoviocytes. In one aspect said nucleic acid delivery vehicle is a virus capsid or a functional part, derivative and/or analogue thereof. Preferably said virus capsid is an adenovirus capsid. Preferably said adenovirus is a subgroup B adenovirus, preferably adenovirus 16. Preferably said tissue tropism is provided by at least a tissue tropism determining part of an adenovirus fiber protein or a functional derivative and/or analogue thereof. The invention further presents methods for the treatment of diseases, preferable joint related diseases.

La présente invention concerne un véhicule d'apport d'acide nucléique doté, spontanément ou non, d'au moins un tropisme pour des cellules ressemblant aux fibroblastes ou aux macrophages, de préférence des cellules synoviales. Pour un aspect de l'invention, ce véhicule d'apport d'acide nucléique est une capside virale, l'une de ses parties fonctionnelles, l'un de ses dérivés et/ou analogues. De préférence, cette capside virale est une capside adénovirale. De préférence, ledit adénovirus est un adénovirus du sous-groupe B, de préférence l'adénovirus 16. Le tropisme tissulaire est conféré par au moins une partie déterminant le tropisme tissulaire d'une protéine adénovirale fibrale, ou de l'un de ses dérivés et/ou analogues fonctionnels. L'invention porte enfin sur des procédures de traitement d'affections, de préférence de nature articulaire.

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