Means and methods for producing a stabilized cell of interest

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/867 (2006.01) C12N 5/071 (2010.01) C12N 5/0781 (2010.01) C07K 14/47 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2575989

The invention provides a method for producing a stabilized cell of interest, said method comprising providing a stem cell and/or a precursor cell of said cell of interest with a nucleic acid sequence which, when present in said cell of interest, is capable of stabilizing said cell of interest, providing a non- human animal with said stem cell and/or precursor cell, allowing generation of said cell of interest in said animal, and obtaining said cell of interest. Said animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.

L'invention concerne un procédé de production d'une cellule stabilisée intéressante. Le procédé comporte les étapes consistant à: fournir à une cellule souche et/ou à une cellule précurseur de la cellule intéressante une séquence d'acide nucléique capable, lorsqu'elle est présente dans ladite cellule intéressante, de stabiliser celle-ci; fournir à un animal non humain ladite cellule souche et/ou la cellule précurseur; faire en sorte que ladite cellule soit produite dans ledit animal; et obtenir ladite cellule intéressante. Ledit animal est de préférence pourvu d'une cellule souche humaine et/ou d'une cellule précurseur humaine permettant de produire une lignée cellulaire humaine.

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