C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/867 (2006.01) C12N 5/071 (2010.01) C12N 5/0781 (2010.01) C07K 14/47 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01) C12P 21/08 (2006.01)
Patent
CA 2575989
The invention provides a method for producing a stabilized cell of interest, said method comprising providing a stem cell and/or a precursor cell of said cell of interest with a nucleic acid sequence which, when present in said cell of interest, is capable of stabilizing said cell of interest, providing a non- human animal with said stem cell and/or precursor cell, allowing generation of said cell of interest in said animal, and obtaining said cell of interest. Said animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.
L'invention concerne un procédé de production d'une cellule stabilisée intéressante. Le procédé comporte les étapes consistant à: fournir à une cellule souche et/ou à une cellule précurseur de la cellule intéressante une séquence d'acide nucléique capable, lorsqu'elle est présente dans ladite cellule intéressante, de stabiliser celle-ci; fournir à un animal non humain ladite cellule souche et/ou la cellule précurseur; faire en sorte que ladite cellule soit produite dans ledit animal; et obtenir ladite cellule intéressante. Ledit animal est de préférence pourvu d'une cellule souche humaine et/ou d'une cellule précurseur humaine permettant de produire une lignée cellulaire humaine.
Berkhout Benjamin
Markusic David Michael
Seppen Jurgen Ewoud
Spits Hergen
Weijer Kees
Academisch Medisch Centrum Bij de Universiteit Van Amsterdam
Borden Ladner Gervais Llp
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