Means and methods for transformation of plant mitochondria

Details Means and methods for transformation of plant mitochondria Means and methods for transformation of plant mitochondria

A - Human Necessities

01

H

A01H 5/00 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2272788

A method is provided for introducing a foreign, exogenously added DNA fragment into mitochondria of plants. Preferably the foreign DNA comprises a DNA fragment allowing replication in mitochondria of plants. The method for producing a transgenic plant or plant cell comprising a foreign DNA fragment in the mitochondria of its cells comprises the steps of a) contacting an untransformed plant cell, preferably derived from a multicellular plant, with the foreign DNA, under conditions allowing uptake of DNA into the plant cell to generate a transgenic plant cell; and b) optionally regenerating a transgenic plant from the transgenic plant cell wherein the foreign DNA comprises an origin of replication for mitochondria and preferably further comprises a gene of interest. Preferably, the gene of interest comprises a promoter region which can direct expression in mitochondria of a plant cell, operably linked to a coding region, and optionally, further operably linked to a transcription terminator region functional in mitochondria. The coding region may comprise one, two or more cistrons.

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