Means for identifying nucleotide sequences involved in apomixis

Details Means for identifying nucleotide sequences involved in apomixis Means for identifying nucleotide sequences involved in apomixis

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2282085

The invention concerns a method for identifying in Gramineae, more particularly in maize, a nucleotide sequence involved in the apomixis in apomictic plants. It is characterised in that it consists in: identifying in the genome of the Gramineae, by phenotypic analysis, genetic mapping and marking by means of transposons, of the meiotic mutations whereof the corresponding gene is shown to be orthologous to genes involved in the expression of apomixis. The invention also concerns the use of a cloned gene in the Gramineae to identify and isolate the orthologous gene sequence in apomictic plants. The invention further concerns the use or modification of the isolated sequence in apomictic forms for inducing an apomictic development in sexual plants.

Le procédé de l'invention pour identifier chez les graminées, et plus spécialement chez le maïs, une séquence de nucléotides impliquée dans l'apomixie chez les plantes apomictiques est caractérisé en ce qu'on identifie dans le génome de la graminée, par analyse phénotypique, cartographie génétique et marquage par l'intermédiaire de transposons, des mutations méiotiques dont on montre que le gène correspondant est orthologue de gènes impliqués dans l'expression de l'apomixie. Utilisation du gène cloné chez la graminée pour identifier et isoler la séquence du gène orthologue chez des plantes apomictiques. Utilisation ou modification de la séquence isolée chez les formes apomictiques pour induire un développement apomictique chez des plantes sexuées.

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