Means for separating lymphocytes and monocytes from...

Details Means for separating lymphocytes and monocytes from... Means for separating lymphocytes and monocytes from...

B - Operations, Transporting

01

D

233/1

B01D 21/26 (2006.01) G01N 33/49 (2006.01)

Patent

CA 1095477

Abstract of the Disclosure This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixo- tropic gel-like material having a specific gravity between 1.065-1.077g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055g/cc.

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