Measurement and use of molecular interactions

G - Physics – 01 – N

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Details

G01N 33/543 (2006.01) G01H 9/00 (2006.01) G01N 27/00 (2006.01) G01N 29/14 (2006.01)

Patent

CA 2369794

A method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprises the steps of: (i) contacting the binding partners, one of which is immobilised on a surface; (ii) oscillating the surface at increasing amplitude; and (iii) detecting a dissociation event. An analogous method can be used to separate a target analyte from a composition. Apparatus for determining the affinity between binding partners, comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and means (14, 15) for detecting a dissociation event.

La présente invention concerne un procédé permettant de mesurer l'affinité existant entre des partenaires de liaison, ou une propriété de l'un des partenaires de liaison dépendant de l'affinité. Ledit procédé consiste à: (i) mettre en contact les partenaires de liaison, dont l'un est immobilisé sur une surface; (ii) faire osciller la surface précitée avec une amplitude croissante; et (iii) détecter un événement de dissociation. On peut utiliser un procédé analogue pour séparer un analyte cible d'une composition. L'invention se rapporte également à un appareil permettant de mesurer l'affinité existant entre des partenaires de liaison, qui comprend: une surface (10) sur laquelle est immobilisé un partenaire de liaison (16) ; un moyen permettant de faire osciller la surface précitée avec une amplitude croissante; et des moyens (14,15) permettant de détecter un événement de dissociation.

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