C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2532220
A quantitative measurement of the progress of a polynucleotide amplification reaction can be made by: (i) carrying out a reaction for the amplification of a target polynucleotide; (ii) either during or after the amplification reaction contacting the amplified product with a molecule that binds to or interacts with a polynucleotide, the molecule being located in a spatially defined position or being determined via a non-linear or non-fluorescent technique; and (iii) detecting the interaction between the amplified product and the molecule by measuring changes in radiation.
Mesure quantitative de l'évolution d'une réaction d'amplification de polynucléotide pouvant consister à : (i) exécuter une réaction visant à amplifier un polynucléotide ciblé ; (ii) soit pendant, soit après la réaction d'amplification, mettre en contact le produit amplifié avec une molécule se liant au polynucléotide ou entrant en interaction avec ce dernier, cette molécule étant située dans une position définie dans l'espace ou déterminée au moyen d'une technique non linéaire ou non fluorescente et (iii) détecter l'interaction entre le produit amplifié et la molécule par mesure des modifications du rayonnement.
Densham Daniel Henry
Torys Llp
LandOfFree
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