G - Physics – 01 – N
Patent
G - Physics
01
N
150/14, 150/15.1
G01N 33/48 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01) G01N 33/86 (2006.01) G01N 33/96 (2006.01)
Patent
CA 1085279
ABSTRACT OF THE DISCLOSURE A method of measuring the level of ethanol in a body fluid includes the step of providing a predetermined volume of body fluid, oxidizing ethanol in the body fluid by the action of an alcohol oxidase in a suitable buffer in the presence of excess molecular oxygen and measuring the rate of oxygen consum- ption, the oxidation taking place in the presence of an agent adapted to suppress the formation of oxygen by peroxide decom- position. Further, a kit for use in the above method comprises (i) a container containing an alcohol oxidase in a suitable buffer, the activity of the oxidase being in the range 1 to 1000 units (as hereindefined) per ml; and (ii) a container containing the agent adapted to suppress the formation of oxygen by peroxide decomposition in a suitable buffer.
269354
Axcell Barry C.
Donninger Cyril
Chembro Holdings (proprietary) Limited
G. Ronald Bell & Associates
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