Measurement of alkaline phosphatase levels in body fluids

Details Measurement of alkaline phosphatase levels in body fluids Measurement of alkaline phosphatase levels in body fluids

G - Physics

01

N

324/25, 150/15.1

G01N 33/48 (2006.01) C12Q 1/42 (2006.01)

Patent

CA 1070227

ABSTRACT OF DISCLOSURE A method of measuring the alkaline phosphatase level in a body fluid such as serum or plasma comprising hydrolysing excess monophosphate ester dissolved in a suitable buffer by action of alkaline phosphatase in a predetermined volume of the body fluid to yield an oxidisable substrate. oxidising the substrate with excess molecular oxygen in the presence of excess oxygenase and measuring the rate of oxygen consumption preferably by means of an oxygen electrode. The invention also provides reagent for use in this method which comprises a diethanolamine-HCl buffer having a molarity of 0,1 M to 4,0 M and a pH of 8.6 to 10.8 and containing magnesium ions in a concentration of 100 to 1000 pM and an excess of oxygenase.

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