Measurement of an insoluble analyte in a sample

Details Measurement of an insoluble analyte in a sample Measurement of an insoluble analyte in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2682196

The present invention relates to compositions, apparatus and methods useful for concurrently performing singular, multiple, high throughput, biological or chemical assays, using nuclease protection molecules which specifically bind to a target of interest. The nuclease protection molecules are capable of detecting targets in complex biological samples, including, preserved, fixed, dried, and/or cross-linked specimen. The reagents and methods of the instant invention provide an effective means for analyzing a target of interest from a complex biological sample without solubilizing or disrupting the sample. Utilization of such methods in clinical and/or diagnostic applications is also described.

La présente invention concerne des compositions, des appareils et procédés utiles pour effectuer simultanément des dosages biologiques ou chimiques simples, multiples, de capacité élevée, utilisant des molécules de protection de nucléase qui se lient spécifiquement à une cible d'intérêt. Les molécules de protection de nucléase sont capables de détecter des cibles dans des échantillons biologiques complexes comprenant un spécimen conservé, fixé, séché et/ou réticulé. Les réactifs et procédés de la présente invention forment un moyen efficace pour analyser une cible d'intérêt à partir d'un échantillon biologique complexe sans solubiliser ou dissocier l'échantillon. L'utilisation de tels procédés dans des applications cliniques et/ou de diagnostic est également décrite.

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