G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/557 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2606548
A method of measuring the rate of binding of a binding substance and an analyte, for example in an assay such as an immunoassay, uses an initial step of performing ultrasonication sufficient to disrupt binding between the binding substance and the analyte. After cessation of the ultrasonication, measurements are taken to determine the rate of binding at cessation of said ultrasonication or at a predetermined time thereafter. The ultrasonication results in knowledge of the precise time of the start of the binding reaction which provides a better rate measurement.
L'invention concerne un procédé de mesure de la vitesse de liaison d'une substance de liaison et d'un analyte, par exemple dans un essai, tel qu'un immunoessai, comprenant une première étape consistant à effectuer une sonication aux ultrasons suffisante pour interrompre la liaison entre la substance de liaison et l'analyte. Suite à la cessation de la sonication aux ultrasons, des mesures sont prises afin de déterminer la vitesse de liaison au moment de la cessation de la sonication aux ultrasons ou à un moment prédéterminé après celle-ci. La sonication aux ultrasons permet d'obtenir le temps précis du début de la réaction de liaison, une meilleure mesure de la vitesse étant ainsi obtenue.
Orion Diagnostica Oy
Robic
LandOfFree
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