Measurement of microbiological activity in an opaque medium

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01) C12Q 1/00 (2006.01) C12Q 1/06 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2443532

A method for monitoring the microbiological contamination of opaque media is described and claimed. In this method, a Fluorogenic Dye is added to an Aliquot of opaque medium. After a certain time period, a fluorometer capable of measuring fluorescent signals in an opaque medium is used to measure the fluoregenic signals of the Fluorogenic Dye and the Reacted Fluorogenic Dye. A Useful RATIO of the fluorescent signal of the Reacted Fluorogenic Dye to the fluorescent signal of the Fluorogenic Dye is calculated and the information gleaned from the RATIO is used to ascertain the state of microbiological contamination in the opaque medium. Figure (1) shows a plot of fluorescent signals, in counts per second, of Resazurin and Resorufin in a Mineral Slurry at Time Zero and at Time Future.

L'invention concerne un procédé permettant de contrôler la contamination microbiologique de milieux opaques. Ce procédé consiste à ajouter un colorant fluorogène à une aliquote de milieu opaque. Après un certain temps, un fluoromètre pouvant mesurer les signaux de fluorescence dans un milieu opaque est utilisé pour mesurer les signaux de fluorescence du colorant fluorogène et du colorant fluorogène soumis à une réaction. Un rapport utile du signal de fluorescence du colorant fluorogène et du signal de fluorescence du colorant fluorogène soumis à une réaction est calculé, et les informations recueillies à partir de ce rapport sont utilisées pour identifier l'état de contamination microbiologique du milieu opaque. L'illustration montre un diagramme de signaux de fluorescence, en désintégration/seconde, de résazurine et de résorufine dans une pâte minérale, à une période zéro et à une période future.

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