Measurement of sperm motility

Details Measurement of sperm motility Measurement of sperm motility

A - Human Necessities

61

B

73/59

A61B 10/00 (2006.01) G01N 21/47 (2006.01) G01P 5/26 (2006.01)

Patent

CA 1201301

ABSTRACT A micro-sample of semen is exposed to a beam of light from an inexpensive and small laser device. Light scattered by the sample is detected by a photodetector. The photodetec- tor signal is analyzed in the frequency domain to provide a measure of the amplitude of the signal components having frequencies above about 100 Hz. This measure is represen- tative of the numbers of motile sperm in the sample. A motile/immotile percentage is obtained by dividing the motile measurement by a value representative of the ampli- tude of the full-frequency photodetector signal. In a preferred embodiment frequency-amplitude analysis is carried out in the time domain using frequency splitting filters and integrating the filtered signals. The light receiving aper- ture for the photodetector is made annular or part-annular in shape to overcome sample orientation or polarization effects.

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