Measuring metabolic rate changes

Details Measuring metabolic rate changes Measuring metabolic rate changes

G - Physics

01

N

G01N 33/497 (2006.01) A01C 1/02 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2403253

The invention relates to methods for measuring metabolic states or rates or changes therein, such as growth rates, dying rates, cell division, metabolite production, and other biological activities of organisms, in particular of small multi-cellular organisms, of seeds and seedlings and of micro-organisms, such as fungi, yeast, bacteria, plant or animal cells and cultures thereof. The invention provides a method for determining a change in metabolic rate of at least one organism comprising placing said organism or part thereof in a confined container and repeatedly or continually measuring the concentration of a metabolic gas in said confined container to determine changes in consumption or production of said gas by said organism wherein said gas concentration is determined without essentially affecting the concentration of said gas in said confined container.

L'invention concerne des procédés destinés à mesurer des états, ou des activités ou des changements métaboliques, tels que vitesse de croissance, taux de mortalité, division cellulaire, production de métabolite, et d'autres activités biologiques d'organismes, en particulier de petits organismes multicellulaires, tels que des champignons, des levures, des bactéries, des cellules végétales ou animales ainsi que de leurs cultures. L'invention concerne un procédé permettant de déterminer un changement d'activité métabolique d'au moins un organisme qui consiste à enfermer cet organisme ou une partie de cet organisme dans un contenant et à mesurer de manière continue ou répétée la concentration d'un gaz métabolique dans ce contenant afin de déterminer les changements de consommation ou de production du contenant et d'obtenir les changements en consommation ou production de ce gaz par l'organisme, la concentration de ce gaz étant déterminée sans affecter sensiblement sa concentration dans le contenant.

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