Measuring method using whole blood sample

Details Measuring method using whole blood sample Measuring method using whole blood sample

G - Physics

01

N

G01N 33/543 (2006.01) G01N 21/76 (2006.01) G01N 33/576 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2172840

A method for measuring a substance to be examined, characterized in that a sufficient amount of a whole blood sample is brought into contact with an insoluble carrier coated with a first partner specifically bindable to said substance to be examined for a short period of time; a complex of said substance to be examined and said first partner is brought into contact with a second partner which is labeled and capable of specifically binding to said first partner, said complex being carried on said insoluble carrier; a resulting complex of said insoluble carrier-said first partner-said substance to be examined-said labeled second partner, and a portion without said resulting complex are separated from eath other; and a signal originating from said label contained in one of said separated portions is detected is disclosed. According to the present invention, the substance to be examined in the whole blood sample can be quickly measured, using the whole blood as a sample without pretreatment.

Une quantité appropriée d'un échantillon de sang entier est maintenue en contact avec un support insoluble recouvert d'une première substance associée pouvant se lier spécifiquement à un objet de mesure pour un court laps de temps. Après que la combinaison de l'objet avec la première substance associée sur le support insoluble est mise en contact avec une seconde substance associée par liaison spécifique à l'objet, le complexe qui en résulte comprenant le support insoluble, la première substance associée, l'objet à mesurer et la seconde substance associée marquée est séparé d'une partie ne contenant pas le complexe. Ensuite, des signaux émanant de la substance marquée contenue dans l'une ou l'autre des parties séparées sont détectés. Selon cette méthode, le sang entier peut être utilisé en tant que tel sans prétraitement de l'échantillon et l'objet à mesurer dans l'échantillon de sang entier peut être mesuré rapidement.

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