Measuring percentage of protein in whole grain samples

G - Physics – 01 – N

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G01N 21/27 (2006.01) G01N 21/35 (2006.01) G01N 33/483 (2006.01) G01N 21/31 (2006.01)

Patent

CA 2067526

Abstract of the Disclosure In a method for measuring protein content of whole grain kernels with a near infrared spectropho- tometer, reflectance measurements are made at wave- lengths shorter than 1600 nanometers. The wavelengths are selected to correspond with absorbance bands of the constituents of the sample. The measurements are encoded into absorbance data and are normalized by subtracting the absorbance value at a first wavelength and then dividing the resulting data by the value at a second wavelength. In accordance with one embodi- ment, the normalized data is substituted in a formula having coefficients determined by regression from samples having known percentages of protein in the sample to yield the percentage of protein in the unknown sample. In accordance with a second embodi- ment, the second differential of the absorbance data is determined and the resulting differential values are inserted in the formula having coefficients obtained from the known samples to yield the percent- age or protein in the unknown sample.

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