Media and methods for culturing plant embryos

Details Media and methods for culturing plant embryos Media and methods for culturing plant embryos

A - Human Necessities

01

H

A01H 4/00 (2006.01)

Patent

CA 2289651

Media and methods are provided for developing plant embryos utilizing low levels of a metabolizable carbon source, generally less than one or two percent. Within certain variants, the invention further subjecting the embryos to water stress during development. The media of the invention comprise less than about 2% of a metabolizable carbon source in combination with sufficient non-permeating osmotica to effect water stressing.

L'invention concerne des milieux et des méthodes qui permettent de cultiver des embryons végétaux avec une source de carbone métabolisable faiblement concentrée, à une concentration généralement inférieure à 1 ou 2 %. Dans certaines variantes, l'invention consiste à soumettre les embryons à des stress hydriques durant leur développement. Les milieux de l'invention contiennent moins de 2 % environ d'une source de carbone métabolisable, associée à des produits osmotiques ne pénétrant pas dans les cellules, en une quantité suffisante pour produire un stress hydrique.

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