Media and methods for promoting maturation of conifer...

Details Media and methods for promoting maturation of conifer... Media and methods for promoting maturation of conifer...

A - Human Necessities

01

H

A01H 4/00 (2006.01) A01H 7/00 (2006.01) C12N 5/02 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2470303

In one aspect, the present invention provides methods for producing mature pine somatic embryos. The methods of the invention comprise the steps of: (a) culturing pine embryonal suspensor mass in a maintenance medium to form pre-cotyledonary pine somatic embryos; (b) culturing the pre-cotyledonary pine somatic embryos formed in step (a) in, or on, a development medium comprising maltose as the principal source of metabolizable sugar and less than 0.5% of gellan gum to form cotyledonary pine somatic embryos; and (c) culturing the cotyledonary pine somatic embryos formed in step (b) in, or on, a maturation medium comprising from about 1% to about 6% sucrose to produce mature pine somatic embryos. In some embodiments, the maturation medium further comprises sucrose and /or abscisic acid.

Dans un aspect, la présente invention a pour objet des méthodes pour produitr des embryons somatiques matures de pin. Les méthodes de la présente invention comprennent les étapes suivantes : a) culture d'une masse de suspenseur embryonnaire de pin dans un milieu de maintenance pour former de embryons somatiques de pin pré-cotylédonnaires; b) culture des embryons somatiques de pin pré-cotylédonnaires formés à l'étape a) dans ou sur un milieu de développement comprenant du maltose comme source principale de sucre métabolisable et moins de 0,5 % de gomme gellane pour former les embryons somatiques cotylédonnaires de pin; c) culture des embryons formés à l'étape b) dans ou sur un milieu de maturation comprenant d'environ 1 à environ 6 % de sucrose pour produire des embryons somatiques matures de pin. Dans certaines réalisations, le milieu de maturation comprend de plus du sucrose et/ou de l'acide abscissique.

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