Media for culturing microorganisms and process for producing...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/14 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2276179

A process for controlling the mycelial morphology of a microorganism belonging to the genus Mortierella during culturing and a process for producing unsaturated fatty acids and a lipid containing them by using a culture medium for culturing a microorganism in which phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the culture medium are in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively, characterized in that the microorganism belonging to the genus Mortierella is cultured in a culture medium containing phosphate ions in the range of 5 to 60 mM, potassium ions in the range of 5 to 60 mM, sodium ions in the range of 2 to 50 mM, magnesium ions in the range of 0.5 to 9 mM, and calcium ions in the range of 0.5 to 12 mM, respectively, to produce unsaturated fatty acids and the lipid containing them, and the culture medium for culturing a microorganism having phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the range of S to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively.

L'invention concerne un procédé destiné à réguler la morphologie mycologique de micro-organismes appartenant au genre Mortierella au moyen d'un milieu de culture de micro-organismes renfermant entre 5 et 60 mM d'ions phosphate, entre 5 et 60 mM d'ions potassium, entre 2 et 50 mM d'ions sodium, entre 0,5 et 9 mM d'ions magnésium et entre 0,5 et 12 mM d'ions calcium; un procédé de production d'acides gras insaturés ou de lipides renfermant ceux-ci, caractérisé par la culture de micro-organismes appartenant au genre Mortierella dans un milieu contenant entre 5 et 60 mM d'ions phosphate, entre 5 et 60 mM d'ions potassium, entre 2 et 50 mM d'ions sodium, entre 0,5 et 9 mM d'ions magnésium et entre 0,5 et 12 mM d'ions calcium; et un milieu de culture de micro-organismes caractérisé par le fait qu'il renferme entre 5 et 60 mM d'ions phosphate, entre 5 et 60 mM d'ions potassium, entre 2 et 50 mM d'ions sodium, entre 0,5 et 9 mM d'ions magnésium et entre 0,5 et 12 mM d'ions calcium.

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