Medium for detecting target microbes in a sample

Details Medium for detecting target microbes in a sample Medium for detecting target microbes in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/04 (2006.01) C12Q 1/10 (2006.01)

Patent

CA 2204121

The invention features a medium for the detection of the presence or absence of a target microbe in a liquified environmental or biological sample comprising: a) an effective amount of a vitamin, an amino acid, an element and a salt ingredients operable to allow viability and reproduction of the tai.get microbe at a rate such that any detectable change in the sample is observable within 18 hours for a sample with less than 10 target microbes per 100 ml; and b) an effective amount of one or more nutrient- indicators provided in an amount sufficient to produce a detectable characteristic signal in the medium during growth of the target microbe; and the nutrient-indicator being operable to alter a detectable characteristic of the sample if metabolized by the target microbe so as to confirm the presence or absence of the target microbe in the sample. The invention also relates to methods for detecting the presence or absence of a target microbe in a liquified environmental or biological sample using the medium.

L'invention concerne un milieu permettant de détecter des microbes cibles dans des milieux liquides provenant de l'environnement ou dans des échantillons biologiques, en moins de 24 heures.

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